sublibrary plasmid Search Results


sublibrary plasmid  (New England Biolabs)
96
New England Biolabs sublibrary plasmid
Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations between replicate sorting experiments for FIX <t>sublibrary</t> tiles (n ≥ 3,144). Pearson’s correlation coefficients are shown.
Sublibrary Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/bio_rxiv__2024__04__01__587474-212-8-21?v=New+England+Biolabs
Average 96 stars, based on 1 article reviews
sublibrary plasmid - by Bioz Stars, 2026-07
96/100 stars
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grna sublibrary  (Addgene inc)
94
Addgene inc grna sublibrary
Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus <t>sublibrary</t> (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.
Grna Sublibrary, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pm37269286-200-201-213?v=Addgene+inc
Average 94 stars, based on 1 article reviews
grna sublibrary - by Bioz Stars, 2026-07
94/100 stars
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genome wide calabrase activation library  (Addgene inc)
94
Addgene inc genome wide calabrase activation library

Genome Wide Calabrase Activation Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pmc11209011-208-0-11?v=Addgene+inc
Average 94 stars, based on 1 article reviews
genome wide calabrase activation library - by Bioz Stars, 2026-07
94/100 stars
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gecko v2 sublibrary b human crispr knockout pooled library  (Addgene inc)
96
Addgene inc gecko v2 sublibrary b human crispr knockout pooled library

Gecko V2 Sublibrary B Human Crispr Knockout Pooled Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pmc11633230__mbio__01925___24___s0001-70-101-117?v=Addgene+inc
Average 96 stars, based on 1 article reviews
gecko v2 sublibrary b human crispr knockout pooled library - by Bioz Stars, 2026-07
96/100 stars
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plasmid pool  (GenScript corporation)
90
GenScript corporation plasmid pool

Plasmid Pool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pm36054348-198-2-7?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
plasmid pool - by Bioz Stars, 2026-07
90/100 stars
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gecko v2 sublibrary  (Addgene inc)
93
Addgene inc gecko v2 sublibrary

Gecko V2 Sublibrary, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pmc11633230__mbio__01925___24___s0001-70-81-97?v=Addgene+inc
Average 93 stars, based on 1 article reviews
gecko v2 sublibrary - by Bioz Stars, 2026-07
93/100 stars
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sublibrary plasmid dna  (Thermo Fisher)
99
Thermo Fisher sublibrary plasmid dna

Sublibrary Plasmid Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pmc00310791-670-0-11?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
sublibrary plasmid dna - by Bioz Stars, 2026-07
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cas9  (Addgene inc)
96
Addgene inc cas9
(A) Schematic depicting the pooled CRISPR-based screen. (B) Gene scores in elesclomol-1-(100 nM) and elesclomol-2-(1uM) treated K562 cells. The gene score is the median log2 fold change in abundance of all sgRNAs targeting that gene during the culture period. The FDX1 score is indicated. (C) The corrected p-values (-log10) of the KS tests of the sgRNA distribution for each gene vs the distribution of all sgRNAs in the screen in the eleslcomol-1 and elesclomol-2 screens. Values are ordered on the x-axis by chromosome and location; the dotted line indicates a corrected p-value of 0.05. The FDX1 score is indicated. (D) Western blot analysis of FDX1 and tubulin (loading control) protein expression levels in WT K562 cells (WT) or cells with FDX1 (two distinct sgRNAs) and AAVS1 deletions. (E-F) Viability curves of parental K562 cells and cells deleted for either AAVS1 (control) or FDX1 achieved with two sgRNAs using <t>CRISPR/Cas9.</t> (E) The indicated cells were treated with increasing concentrations of eleslcomol-1 and viability was examined after 72 hours. (F) The indicated cells were grown in the presence of either glucose or galactose and the relative cell number plotted.
Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/bio_rxiv__288365-219-13-15?v=Addgene+inc
Average 96 stars, based on 1 article reviews
cas9 - by Bioz Stars, 2026-07
96/100 stars
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qiafilter maxi  (Qiagen)
96
Qiagen qiafilter maxi
(A) Schematic depicting the pooled CRISPR-based screen. (B) Gene scores in elesclomol-1-(100 nM) and elesclomol-2-(1uM) treated K562 cells. The gene score is the median log2 fold change in abundance of all sgRNAs targeting that gene during the culture period. The FDX1 score is indicated. (C) The corrected p-values (-log10) of the KS tests of the sgRNA distribution for each gene vs the distribution of all sgRNAs in the screen in the eleslcomol-1 and elesclomol-2 screens. Values are ordered on the x-axis by chromosome and location; the dotted line indicates a corrected p-value of 0.05. The FDX1 score is indicated. (D) Western blot analysis of FDX1 and tubulin (loading control) protein expression levels in WT K562 cells (WT) or cells with FDX1 (two distinct sgRNAs) and AAVS1 deletions. (E-F) Viability curves of parental K562 cells and cells deleted for either AAVS1 (control) or FDX1 achieved with two sgRNAs using <t>CRISPR/Cas9.</t> (E) The indicated cells were treated with increasing concentrations of eleslcomol-1 and viability was examined after 72 hours. (F) The indicated cells were grown in the presence of either glucose or galactose and the relative cell number plotted.
Qiafilter Maxi, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/us09428572-566-14-13?v=Qiagen
Average 96 stars, based on 1 article reviews
qiafilter maxi - by Bioz Stars, 2026-07
96/100 stars
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sublibrary guide vector  (Addgene inc)
93
Addgene inc sublibrary guide vector
(A) Schematic depicting the pooled CRISPR-based screen. (B) Gene scores in elesclomol-1-(100 nM) and elesclomol-2-(1uM) treated K562 cells. The gene score is the median log2 fold change in abundance of all sgRNAs targeting that gene during the culture period. The FDX1 score is indicated. (C) The corrected p-values (-log10) of the KS tests of the sgRNA distribution for each gene vs the distribution of all sgRNAs in the screen in the eleslcomol-1 and elesclomol-2 screens. Values are ordered on the x-axis by chromosome and location; the dotted line indicates a corrected p-value of 0.05. The FDX1 score is indicated. (D) Western blot analysis of FDX1 and tubulin (loading control) protein expression levels in WT K562 cells (WT) or cells with FDX1 (two distinct sgRNAs) and AAVS1 deletions. (E-F) Viability curves of parental K562 cells and cells deleted for either AAVS1 (control) or FDX1 achieved with two sgRNAs using <t>CRISPR/Cas9.</t> (E) The indicated cells were treated with increasing concentrations of eleslcomol-1 and viability was examined after 72 hours. (F) The indicated cells were grown in the presence of either glucose or galactose and the relative cell number plotted.
Sublibrary Guide Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pm38242867-337-17-22?v=Addgene+inc
Average 93 stars, based on 1 article reviews
sublibrary guide vector - by Bioz Stars, 2026-07
93/100 stars
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sublibraries  (Addgene inc)
90
Addgene inc sublibraries
(A) Schematic depicting the pooled CRISPR-based screen. (B) Gene scores in elesclomol-1-(100 nM) and elesclomol-2-(1uM) treated K562 cells. The gene score is the median log2 fold change in abundance of all sgRNAs targeting that gene during the culture period. The FDX1 score is indicated. (C) The corrected p-values (-log10) of the KS tests of the sgRNA distribution for each gene vs the distribution of all sgRNAs in the screen in the eleslcomol-1 and elesclomol-2 screens. Values are ordered on the x-axis by chromosome and location; the dotted line indicates a corrected p-value of 0.05. The FDX1 score is indicated. (D) Western blot analysis of FDX1 and tubulin (loading control) protein expression levels in WT K562 cells (WT) or cells with FDX1 (two distinct sgRNAs) and AAVS1 deletions. (E-F) Viability curves of parental K562 cells and cells deleted for either AAVS1 (control) or FDX1 achieved with two sgRNAs using <t>CRISPR/Cas9.</t> (E) The indicated cells were treated with increasing concentrations of eleslcomol-1 and viability was examined after 72 hours. (F) The indicated cells were grown in the presence of either glucose or galactose and the relative cell number plotted.
Sublibraries, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/pm36409314-164-14-8?v=Addgene+inc
Average 90 stars, based on 1 article reviews
sublibraries - by Bioz Stars, 2026-07
90/100 stars
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sublibrary plasmid  (Addgene inc)
93
Addgene inc sublibrary plasmid
(A) Schematic depicting the pooled CRISPR-based screen. (B) Gene scores in elesclomol-1-(100 nM) and elesclomol-2-(1uM) treated K562 cells. The gene score is the median log2 fold change in abundance of all sgRNAs targeting that gene during the culture period. The FDX1 score is indicated. (C) The corrected p-values (-log10) of the KS tests of the sgRNA distribution for each gene vs the distribution of all sgRNAs in the screen in the eleslcomol-1 and elesclomol-2 screens. Values are ordered on the x-axis by chromosome and location; the dotted line indicates a corrected p-value of 0.05. The FDX1 score is indicated. (D) Western blot analysis of FDX1 and tubulin (loading control) protein expression levels in WT K562 cells (WT) or cells with FDX1 (two distinct sgRNAs) and AAVS1 deletions. (E-F) Viability curves of parental K562 cells and cells deleted for either AAVS1 (control) or FDX1 achieved with two sgRNAs using <t>CRISPR/Cas9.</t> (E) The indicated cells were treated with increasing concentrations of eleslcomol-1 and viability was examined after 72 hours. (F) The indicated cells were grown in the presence of either glucose or galactose and the relative cell number plotted.
Sublibrary Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sublibrary+plasmid/bio_rxiv__2025__09__15__676433-245-14-16?v=Addgene+inc
Average 93 stars, based on 1 article reviews
sublibrary plasmid - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

Image Search Results


Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations between replicate sorting experiments for FIX sublibrary tiles (n ≥ 3,144). Pearson’s correlation coefficients are shown.

Journal: bioRxiv

Article Title: Multiplex, multimodal mapping of variant effects in secreted proteins

doi: 10.1101/2024.04.01.587474

Figure Lengend Snippet: Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations between replicate sorting experiments for FIX sublibrary tiles (n ≥ 3,144). Pearson’s correlation coefficients are shown.

Article Snippet: To barcode each sublibrary, 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C, then heat-inactivated at 65°C for 20 minutes.

Techniques: Functional Assay

Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations for variants that are shared between sublibrary tiles (top row: n = 397, bottom row: n = 380). Pearson’s correlation coefficients are shown.

Journal: bioRxiv

Article Title: Multiplex, multimodal mapping of variant effects in secreted proteins

doi: 10.1101/2024.04.01.587474

Figure Lengend Snippet: Pairwise MultiSTEP secretion and γ-carboxylation functional score correlations for variants that are shared between sublibrary tiles (top row: n = 397, bottom row: n = 380). Pearson’s correlation coefficients are shown.

Article Snippet: To barcode each sublibrary, 1 μg of each sublibrary plasmid was digested at 37°C for 5 hours with NheI-HF and SacI-HF (New England Biolabs), incubated with rSAP for 30 minutes at 37°C, then heat-inactivated at 65°C for 20 minutes.

Techniques: Functional Assay

Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus sublibrary (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.

Journal: Cell reports

Article Title: Host genetics highlights IFN-γ-dependent Toxoplasma genes encoding secreted and non-secreted virulence factors in in vivo CRISPR screens.

doi: 10.1016/j.celrep.2023.112592

Figure Lengend Snippet: Figure 7. The UFMylation pathway contributes to Toxoplasma virulence (A) Rank-ordered plots for in vivo fitness scores from the endomembrane-nucleus sublibrary (left) and the metabolism sublibrary (right). UFMylation-related genes are marked in red. Error bars (gray) represent SEM. (B) Fitness scores of the UFMylation-related genes in Vero cells (in vitro), WT mice (WT), and Ifngr1/ mice (KO) are shown as box plots. Each dot represents fitness scores for individual gRNAs. ***p < 0.001; *p < 0.05 (Wilcoxon rank-sum test). (C) The conserved C-terminal amino acid sequences of the UFM1 homologs from indicated apicomplexan (blue) and model organisms (black). The conserved glycine residue is shown by the arrow. (D) Survival curves of WT mice with intra-footpad infection of 103 tachyzoites of WT (n = 10), DUFM1 (n = 6), DUFM1 + UFM1 (WT) (n = 7), and DUFM1 + UFM1 (G87A) (n = 7). ***p < 0.001; N.S., not significant (log-rank test). (E) Complementations of FLAG-tagged WT and G87A UFM1 protein in DUFM1 parasites. (F) Survival curves of WT (n = 10) or Ifngr1/ (n = 9) mice with intra-footpad infection of 103 tachyzoites of DUFM1 parasites. ***p < 0.001 (log-rank test). See also Figure S6.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_203160 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGST2 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DEPT1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DRAB4 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DHMGB This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DALG2 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DDGAT1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_211695 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DPDX1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DPDX2 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DEF-P This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_204350 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DTGGT1_215890 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUSPase This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DRad23 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DHID1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DSui1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGRA17 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGRA72+ GRA72-HA This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/ GRA23-HA This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DGRA72/GRA23-HA This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUFM1 This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUFM1+UFM1(WT) This study N/A T. gondii: Strain RH/Dhxgprt/Dku80/DUFM1+UFM1(G87A) This study N/A C57BL/6 mice SLC N/A Ifngr1 / mice Sasai et al.71 N/A Oligonucleotides For primers and oligonucleotides, see Table S4 This study N/A Recombinant DNA For each gRNA sublibrary, see Tables S1–S3 This study N/A pU6-Universal Sidik et al.72 Addgene plasmid: #52694 pSAG1-GRA72-HA-HXGPRT This study N/A pUPRT-UFM1(WT) This study N/A pUPRT-UFM1(G87A) This study N/A (Continued on next page) Cell Reports 42, 112592, June 27, 2023 17

Techniques: In Vivo, In Vitro, Residue, Infection

Journal: iScience

Article Title: CRISPR-Cas9 screening reveals a distinct class of MHC-I binders with precise HLA-peptide recognition

doi: 10.1016/j.isci.2024.110120

Figure Lengend Snippet:

Article Snippet: Genome-wide Calabrase activation library (sublibrary A+B) , Dr. J. Doench , Addgene cat: 1000000111.

Techniques: Virus, Recombinant, Blocking Assay, Genome Wide, Activation Assay, CRISPR, Knock-Out, Mutagenesis, Plasmid Preparation, Software, Imaging

(A) Schematic depicting the pooled CRISPR-based screen. (B) Gene scores in elesclomol-1-(100 nM) and elesclomol-2-(1uM) treated K562 cells. The gene score is the median log2 fold change in abundance of all sgRNAs targeting that gene during the culture period. The FDX1 score is indicated. (C) The corrected p-values (-log10) of the KS tests of the sgRNA distribution for each gene vs the distribution of all sgRNAs in the screen in the eleslcomol-1 and elesclomol-2 screens. Values are ordered on the x-axis by chromosome and location; the dotted line indicates a corrected p-value of 0.05. The FDX1 score is indicated. (D) Western blot analysis of FDX1 and tubulin (loading control) protein expression levels in WT K562 cells (WT) or cells with FDX1 (two distinct sgRNAs) and AAVS1 deletions. (E-F) Viability curves of parental K562 cells and cells deleted for either AAVS1 (control) or FDX1 achieved with two sgRNAs using CRISPR/Cas9. (E) The indicated cells were treated with increasing concentrations of eleslcomol-1 and viability was examined after 72 hours. (F) The indicated cells were grown in the presence of either glucose or galactose and the relative cell number plotted.

Journal: bioRxiv

Article Title: Inhibition of mitochondrial ferredoxin 1 (FDX1) prevents adaptation to proteotoxic stress

doi: 10.1101/288365

Figure Lengend Snippet: (A) Schematic depicting the pooled CRISPR-based screen. (B) Gene scores in elesclomol-1-(100 nM) and elesclomol-2-(1uM) treated K562 cells. The gene score is the median log2 fold change in abundance of all sgRNAs targeting that gene during the culture period. The FDX1 score is indicated. (C) The corrected p-values (-log10) of the KS tests of the sgRNA distribution for each gene vs the distribution of all sgRNAs in the screen in the eleslcomol-1 and elesclomol-2 screens. Values are ordered on the x-axis by chromosome and location; the dotted line indicates a corrected p-value of 0.05. The FDX1 score is indicated. (D) Western blot analysis of FDX1 and tubulin (loading control) protein expression levels in WT K562 cells (WT) or cells with FDX1 (two distinct sgRNAs) and AAVS1 deletions. (E-F) Viability curves of parental K562 cells and cells deleted for either AAVS1 (control) or FDX1 achieved with two sgRNAs using CRISPR/Cas9. (E) The indicated cells were treated with increasing concentrations of eleslcomol-1 and viability was examined after 72 hours. (F) The indicated cells were grown in the presence of either glucose or galactose and the relative cell number plotted.

Article Snippet: 268M cells were transduced with 22mL human genome-wide cleavage-optimized lentiviral sgRNA library containing Cas9 ( http://www.addgene.org/pooled-library/sabatini-crispr-human-high-activity-3-sublibraries ) as previously described ( ).

Techniques: CRISPR, Western Blot, Expressing